Syd Labs重组抗小鼠PD-1小鼠IgG2a Kappa单克隆抗体产品说明

Syd Labs体内实验级重组抗小鼠PD-1小鼠IgG2a Kappa单克隆抗体（克隆号29F.1A12.1）（In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2a Kappa Monoclonal Antibody （Clone 29F.1A12.1））。

用哺乳动物细胞生产的重组抗小鼠PD1单克隆抗体（Recombinant anti-mouse PD1 monoclonal antibodies）与大鼠抗小鼠PD-1 / CD279单克隆抗体（rat anti-mouse PD-1 / CD279 monoclonal antibody）（克隆号:29F.1A12）具有相同的可变区序列，适合体外和体内研究。

重点说明：此产品仅限于科研使用，不得用于人类或动物的诊断或治疗。

多找找 | Syd Labs体内实验级重组抗小鼠PD-1小鼠IgG2a Kappa单克隆抗体产品参数

cat#：PA007163.m2a

产品名称：体内实验级重组抗小鼠PD-1小鼠IgG2a Kappa单克隆抗体(克隆号29F.1A12.1)

英文名：In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2a Kappa Monoclonal Antibody （Clone 29F.1A12.1）

供货商名称：Syd Labs, Inc.

公司名：Syd Labs

别称：程序性细胞死亡蛋白1，PD-1，CD279，分化簇279，29F.1A12

概述：用哺乳动物细胞生产的体内实验级重组抗小鼠PD-1小鼠IgG2a Kappa单克隆抗体（克隆号29F.1A12.1）（In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2a Kappa Monoclonal Antibody （Clone 29F.1A12.1））。

克隆号：29F.1A12.1，与大鼠抗小鼠PD-1单克隆抗体（rat anti-mouse PD-1 monoclonal antibody）（克隆号:29F.1A12）相同的可变区序列。

同种型：大鼠 IgG2a, kappa

应用：western blot (WB)、免疫组织化学(IHC)、流式细胞术(FC)以及各种体外和体内功能检测。

免疫源：用小鼠PD-1 cDNA免疫大鼠，再用小鼠PD-1- Ig融合蛋白免疫大鼠，产生原大鼠杂交瘤(克隆号:29F.1A12)。

抗体形式：0.2 μM过滤溶液，1x PBS

内毒素：根据 LAL 方法，≤1 EU每1mg 蛋白质。提供特级体内实验级重组抗小鼠PD-1小鼠IgG2a Kappa单克隆抗体（克隆号29F.1A12.1）（In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2a Kappa Monoclonal Antibody （Clone 29F.1A12.1），内毒素≤0.05 EU/mg)。

纯度：>95%（在还原条件下通过SDS-PAGE测定）

运输：体内实验级重组抗小鼠PD-1小鼠IgG2a Kappa单克隆抗体（克隆号29F.1A12.1）（In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2a Kappa Monoclonal Antibody （Clone 29F.1A12.1））用冰袋运输。收到后，请立即将其存放在下面建议的温度下。

稳定性与存储：使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，如果保存在2 至 8°C。 自收到之日起 3 个月，如果保存在-20 至 -70°C。

注意事项：最便宜的体内实验级重组人IgG1 kappa同型对照抗体和突变体（In Vivo Grade Recombinant Human IgG1 kappa Isotype Control Antibody mutant）;与经测试的人体样本的特异性结合程度低或无特异性结合;人源化可变区和人IgG1 kappa恒定区;可提供嵌合版本和优质级(内毒素≤0.05 EU/mg)。

PA007163.m2a: 体内实验级重组抗小鼠PD-1小鼠IgG2a Kappa单克隆抗体（克隆号29F.1A12.1）（In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2a Kappa Monoclonal Antibody （Clone 29F.1A12.1））

背景知识

The rat anti-mouse PD-1 monoclonal antibody（大鼠抗小鼠PD-1单克隆抗体） 29F.1A12 (rat IgG2a kappa) reacts with the mouse PD-1 protein (programmed death-1 or CD279) encoded by the mouse Pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 29F.1A12 monoclonal antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein.

Our recombinant 29F.1A12 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 monoclonal antibody（大鼠抗小鼠PD-1单克隆抗体） （hybridoma clone name or number: 29F.1A12）.

Questions and Answers about anti-mouse PD-1 antibody (Clone 29F.1A12):

Question: Which anti-mouse PD1 antibody（抗小鼠PD-1抗体） works the best?
Answer: (by AI) There are several anti-mouse PD1 antibodies（抗小鼠PD1抗体） available in the market. Bio X Cell offers three different anti-mouse PD-1 monoclonal antibodies（抗小鼠PD-1单克隆抗体）: Clones RMP1-14, 29F.1A12, and J43. All three of these antibodies are commonly used to block PD-1 signaling in vivo in murine tumor models and other mouse models. These three clones all have extensive multi-year publication records supporting them. Leinco Technologies offers four different anti-PD-1 monoclonal antibodies（抗PD-1单克隆抗体）: 29F.1A12, RMP1-14, J43, and 5C4-B83. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells). (by Syd Labs) Syd Labs offers anti-mouse PD-1 / CD279 monoclonal antibodies（抗小鼠PD-1 / CD279单克隆抗体） based on the sequences of clones RMP1-14 and 29F.1A12. Syd Labs provides in vivo grade recombinant antibodies（体内实验级重组抗体） including engineered antibodies for the clones RMP1-14 and 29F.1A12.

Question: Do you produce Fc-silenced 29F.1A12 antibody?
Answer: We provide recombinant 29F.1A12 antibodies including the Fc-silenced or Fc silent versions of recombinant 29F.1A12 antibodies. It is very easy for us to produce the Fc-silenced or Fc silent versions of recombinant 29F.1A12 antibodies no matter which isotypes they are based, for example, mIgG2c LALAPG.

Question: What are the published applications of the 29F.1A12 antibody?
Answer: It was reported that the blockade of the PD-1 protein expressed on TME-infiltrating macrophages and natural killer (NK) cells by 29F.1A12 results in tumor growth suppression and prolonged survival. It is also reported that the 29F.1A12 antibody recognizes the PD-1 surface protein on B16-F10 and other murine tumor lines, suppresses cancer cell-intrinsic PD-1 receptor signaling, and inhibits resultant tumor growth in three-dimensional (3D) culture and tumor-bearing mice. In the paper “Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells”, it was shown that the anti-PD-1 29F.1A12 monoclonal antibody（抗PD-1 29F.1A12单克隆抗体） recognizes the PD-1 surface protein on live B16-F10 melanoma cells, live YUMM melanoma variant lines, and overlapping WT murine B16-F10 and YUMM melanoma and T-cell subsets by FACS. In contrast to the RMP1-30 antibody, The 29F.1A12 PD-1 blocking antibody inhibits PD-1 interactions with PD-L1 on live B16-F10 melanoma cells. The RMP1-14 clone, but not 29F.1A12 or RMP1-30, is useful for pulling down (IP) and sequencing PD-1 from B16-F10 and T-cell cultures, consistent with the previous observations that individual antibodies differ in their suitability for specific assay types. 29F.1A12 binding to B16-F10 cells was increased in 3D versus 2D settings.

Syd Labs体内实验级重组抗小鼠PD-1小鼠IgG2a Kappa单克隆抗体（克隆号29F.1A12.1）（In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2a Kappa Monoclonal Antibody （Clone 29F.1A12.1））（PA007163.M2A）更多的产品技术信息，来源于多找找科技。