云南
Product Profile
Product Name
GRP78 antibody
Antibody Type
Primary Antibodies
Product description
Rabbit Polyclonal antibody to GRP78GRP78 (HSPA5), also referred to as ＇immunoglobulin heavy chain-binding protein＇ (BiP), is a member of the heat-shock protein-70 (HSP70) family ａnd is involved in the folding ａnd assembly of proteins in the endoplasmic reticulum (ER). Because so many ER pr
Immunogen
Synthetic peptide made to an internal portion of the human protein (within residues 250-300). [Swiss-Prot# P11021]
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
FACSICC/IFWB
Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity
HumanMouseChickenRatSheep
Predicted Reactivity
Bovine, C. elegans, Drosophila, Primate, ZebrafishPrimate (100%), Bovine (100%), Zebrafish (100%), Drosophila (100%), C. elegans (100%)
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
More
BIP
FLJ26106
MIF2
GRP78
Target
HSPA5
Tissue Specificity
Grp78
Cellular Localization
Endoplasmic reticulum lumen. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Application
Application
- Simple Western: GRP78/HSPA5 Antibody [ - Simple Western lane view shows a specific band for GRP78 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Application
- Detection of Bip/Grp78 on HeLa whole cell extracts using
Application Notes
Recommended Starting Dilutions: For WB: Use at a concentration of 0.5 ug/ml. For FACS: Use at a dilution of 1:150. For ICC/IF: Use at a dilution of 1:50. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at 4C short term. Aliquot ａnd store at -20C long term. Avoid freeze-thaw cycles.
Storage Buffer
PBS, 30% glycerol ａnd 0.1% sodium azide was added as a preservative.
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