推荐：Cefixime Tellurite Supplement@2024已更新

别名:E. coli Selective Supplement, Escherichia coli Selective Supplement, CT supplement

属性
质量水平
200
形式
solid
组成
Cefixime, 0.0025 mg per vial
Potassium tellurite, 1.25 mg per vial
包装
pkg of 10 vials
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
相容性
GranuCult® prime Sorbitol MacConkey (SMAC) Agar (1.00213)
储存温度
2-8°C
适用性
Escherichia coli

说明
一般描述
CT-Supplement contains cefixime and potassium tellurite. It is used in combination with Sorbitol MacConkey Agar (SMCA) to enhance the isolation of Escherichia coli O157.
应用
CT-Supplement is an additive for the preparation of CT-SMAC Agar for the isolation and differentiation of enterohemorrhagic (EHEC) E. coli 0157:H7-strains from foodstuffs and stool.
联系
Replaces: 1092020017
分析说明
Appearance
- clearness:
clear
- colour: colourless
pH-value (25 °C): 5.3 - 5.7
Typical composition (per vial): Cefixim 0.025 mg; Potassium Tellurite 1.25 mg.
Growth promotion test in accordance with ISO 11133.
Supplement tested with base Art. 1.09207.
Inoculum on reference medium
- Escherichia coli ATCC 35150 (O157):
10 - 100
- Escherichia coli O157:H7 ATCC 43894: 10 - 100
- Escherichia coli O157:H7 ATCC 43895: 10 - 100
Inoculum
- Escherichia coli ATCC 11775 (WDCM 00090):
>10⁴
- Escherichia coli ATCC 25922 (WDCM 00013): >10⁴
- Staphylococcus aureus ATCC 6538 (WDCM 00032): >10⁴
- Staphylococcus aureus ATCC 25923 (WDCM 00034): >10⁴
Recovery on test medium
- Escherichia coli ATCC 35150 (O157):
≥ 50%
- Escherichia coli O157:H7 ATCC 43894: ≥ 50%
- Escherichia coli O157:H7 ATCC 43895: ≥ 50%
Growth
- Escherichia coli ATCC 11775 (WDCM 00090):
partly inhibition
- Escherichia coli ATCC 25922 (WDCM 00013): partly inhibition
- Staphylococcus aureus ATCC 6538 (WDCM 00032): total inhibition
- Staphylococcus aureus ATCC 25923 (WDCM 00034): total inhibition
Colony colour
- Escherichia coli ATCC 35150 (O157):
colourless
- Escherichia coli O157:H7 ATCC 43894: colourless
- Escherichia coli O157:H7 ATCC 43895: colourless
- Escherichia coli ATCC 11775 (WDCM 00090): pink
- Escherichia coli ATCC 25922 (WDCM 00013): pink
Incubation: 24 hrs.; 37 °C; aerobic.
Reference: Tryptic-soy-agar. Inhibited strains tested with three-loop-streak.

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