J Agric Food Chem：体外表没食子儿茶素-3-没食子酸酯（EGCG）与人体肠道菌群的相互作用研究

茶酚类物质与肠道菌群的相互作用可能在这些生物活性化合物的健康益处中发挥了不可或缺的作用，但这种相互作用尚未明确。荷兰瓦赫宁根大学食品化学实验室的Zhibin Liu、Wouter J. C. de Bruijn和Jean-Paul Vincken*等人通过体外发酵的方法，对表没食子儿茶素-3-没食子酸酯(EGCG)的代谢归宿及其对肠道菌群的影响进行了全面的研究。

超高效液相色谱-四极杆-静电场轨道阱高分辨质谱（ultrahigh performance liquid chromatography hybrid quadrupole Orbitrap mass spectrometry，UHPLC-Q-Orbitrap-MS）分析显示，EGCG通过连续的酯水解、C环开环、A环裂变、脱羟基和脂肪链缩短而迅速分解成一系列代谢产物，包括4-苯基丁酸、3-（3'，4'-二羟基苯基）丙酸、3-（4'-羟苯基）丙酸。微生物组分析表明，与空白对照相比，EGCG处理可刺激有益的拟杆菌(Bacteroides)、克里斯滕森菌科（Christensenellaceae）和双歧杆菌(Bifidobacterium)。此外，致病菌变形梭杆菌（Fusobacterium varium）、嗜胆菌（Bilophila）、肠杆菌科（Enterobacteriaceae）均受到抑制。而且，代谢产物浓度的变化（包括4-苯基丁酸和苯乙酸）与特定肠道菌群的丰度变化密切相关。EGCG和肠道菌群之间的相互作用可能共同促进了EGCG的健康益处。

Abstract

Reciprocal Interactions between Epigallocatechin-3-gallate (EGCG) and Human Gut Microbiota In Vitro

Zhibin Liu, Wouter J. C. de Bruijn, Marieke E. Bruins, and Jean-Paul Vincken

Interaction of tea phenolics with gut microbiota may play an integral role in the health benefits of these bioactive compounds, yet this interaction is not fully understood. Here, the metabolic fate of epigallocatechin-3-gallate (EGCG) and its impact on gut microbiota were integrally investigated viain vitro fermentation. As revealed by ultrahigh performance liquid chromatography hybrid quadrupole Orbitrap mass spectrometry (UHPLC-Q-Orbitrap-MS), EGCG was promptly degraded into a series of metabolites, including 4-phenylbutyric acid, 3-(3′,4′-dihydroxyphenyl)propionic acid, and 3-(4′-hydroxyphenyl)propionic acid, through consecutive ester hydrolysis, C-ring opening, A-ring fission, dehydroxylation, and aliphatic chain shortening. Microbiome profiling indicated that, compared to the blank, EGCG treatment resulted in stimulation of the beneficial bacteria Bacteroides, Christensenellaceae, and Bifidobacterium. Additionally, the pathogenic bacteria Fusobacterium varium, Bilophila, and Enterobacteriaceae were inhibited. Furthermore, changes in concentrations of metabolites, including 4-phenylbutyric acid and phenylacetic acid, were strongly correlated with changes in the abundance of specific gut microbiota. These reciprocal interactions between EGCG and gut microbiota may collectively contribute to the health benefits of EGCG.

该文章《Reciprocal Interactions between Epigallocatechin-3-gallate (EGCG) and Human Gut Microbiota In Vitro》于《Journal of Agricultural and Food Chemistry》2020年08月出版。

为进一步促进动物源食品科学的发展，带动产业的技术创新，更好的保障人类身体健康和提高生活品质，北京食品科学研究院和中国食品杂志社在成功召开“2019年动物源食品科学与人类健康国际研讨会（宁波）”的基础上，将与青海大学农牧学院于2020年10月22-23日在西宁共同举办“2020年动物源食品科学与人类健康国际研讨会”。研讨会将就肉、水产、禽蛋、乳制品等动物源食品科学基础研究、现代化加工技术，贮藏、保鲜及运输，质量安全与检测技术，营养及风味成分分析，副产物综合利用，法律、法规及发展政策等方面的重大理论研究展开深入探讨，交流和借鉴国外经验，为广大食品科研工作者和生产者提供新的思路，指明发展方向。