NQO1 Antibody (Center) Catalog Number：OM215597

Product Profile

Product Name

NQO1 Antibody (Center)

Antibody Type

Primary Antibodies

Key Feature

Clonality

Polyclonal

Isotype

Ig

Host Species

Rabbit

Tested Applications

WBFCIF

WB~~1:50~100 FC~~1:10~50 IF~~1:10~50:

Species Reactivity

Human

Concentration

1 mg/ml

Purification

Target Information

Gene Synonyms

DIA4
NMOR1

Alternative Names

More

NQO1
DIA4
NMOR1
NAD(P)H dehydrogenase [qui] 1
Azoreductase
DT-diaphorase
Menadione reductase
NAD(P)H:qui oxidoreductase 1
Phylloqui reductase
Qui reductase 1

Molecular Weight(MW)

30868 Da

Function

The enzyme apparently serves as a qui reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis

Tissue Specificity

This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the Center region of human NQO1.

Cellular Localization

Cylasm.

Database Links

Entrez Gene

1728

Application

Application

• Western blot analysis of NQO1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab.

Application

• NQO1 antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell ( histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Application

• Confocal immunofluorescent analysis of NQO1 Antibody (Center)(Cat#AP7350c) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Application Notes

WB~~1:50~100 FC~~1:10~50 IF~~1:10~50:

Additional Information

Form

Liquid

Storage Instructions

For short-term storage, store at 4° C. For long-term storage, aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Storage Buffer

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.