Grp78 antibody , Internal Catalog Number：OM255580

Product Profile

Product Name

Grp78 antibody , Internal

Antibody Type

Primary Antibodies

Product description

More

Rabbit polyclonal antibody to Grp78 (heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa))When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as ＇immunoglobulin heavy chain-binding protein＇ (BiP), is a member of the heat-shock protein-70 (HSP70) family ａnd is involved in the folding ａnd assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

Key Feature

Clonality

Polyclonal

Isotype

IgG

Host Species

Rabbit

Tested Applications

ICC/IFIHC-PIPWB

More

WB:1:100~1:400
IP:1:20~1:100
ICC/IF:1:20~1:100
IHC-p:1:10~1:50

Species Reactivity

HumanMouseRat

Predicted Reactivity

Zebrafish, Japanese medaka, Xenopus laevis, Chicken, Rhesus Monkey, BovineZebrafish (98%), Japanese medaka (97%), Xenopus laevis (97%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)

Concentration

0.2 mg/ml

Purification

Affinity purified

Target Information

Gene Synonyms

More

BIP antibody
FLJ26106 antibody
GRP78 antibody
MIF2 antibody
HSPA5 antibody
immunoglobulin heavy chain-binding protein antibody
78 kDa glucose-regulated protein antibody
endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody

Target

Grp78

Molecular Weight(MW)

72 kDa (note)

Tissue Specificity

Knockdown/Knockout validation was supported by customer review data.

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Application

Application

• Non-transfected (–) ａnd transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, ａnd the membrane was blotted with Grp78 antibody [N2C1], Internal diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Application

• Grp78 antibody [N2C1], Internal detects Grp78 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody [N2C1], Internal diluted at 1:100. Blue: Hoechst 33342 staining.

Application

• Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal . Western blot analysis was performed using Grp78 antibody [N2C1], Internal . EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Application

• Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-em
• bedded mouse intestine. Grp78 antibody [N2C1], Internal dilution: 1:50.

Application

• Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-em
• bedded rat hind brain. Grp78 antibody [N2C1], Internal dilution: 1:50.

Application

• Grp78 antibody [N2C1], Internal detects Grp78 protein at cylasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-em
• bedded mouse brain. Grp78 antibody [N2C1], Internal diluted at 1:50.

Application

• Grp78 antibody [N2C1], Internal detects Grp78 protein at cylasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-em
• bedded rat prostate. Grp78 antibody [N2C1], Internal diluted at 1:50.

Application

• Grp78 antibody detects Grp78 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, ａnd the membrane was blotted with Grp78 antibody diluted at a dilution of 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Application Notes

More

WB:1:100~1:400
IP:1:20~1:100
ICC/IF:1:20~1:100
IHC-p:1:10~1:50

Additional Information

Form

Liquid

Storage Instructions

Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Storage Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.