GCLM antibody Catalog Number：OM254276

Product Profile

Product Name

GCLM antibody

Antibody Type

Primary Antibodies

Product description

More

Rabbit Polyclonal antibody to GCLM (glutamate-cysteine ligase, modifier subunit)Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit ａnd a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCLM. The exact sequence is proprietary.

Key Feature

Clonality

Polyclonal

Isotype

IgG

Host Species

Rabbit

Tested Applications

ICC/IFWB

*Optimal dilutions/concentrations should be determined by the researcher.:

Species Reactivity

HumanMouseRat

Predicted Reactivity

BovineBovine (96%)

Concentration

1 mg/ml

Purification

Affinity purified

Target Information

Gene Synonyms

More

GLCLR antibody
GCLM antibody
GSC light chain antibody
gamma-ECS regulatory subunit antibody
GCS light chain antibody
"glutamate-cysteine ligase (gamma-glutamylcysteine synthetase)
regulatory (30.8kD) antibody"
glutamate－－cysteine ligase modifier sub

Molecular Weight(MW)

31 kDa (note) (2)

Application

Application

• Sample (50 µg of whole cell lysate) A: rat liver 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Application

• Sample (30 µg of whole cell lysate) A: A431 12% SDS PAGE GCLM antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Application

• Immunofluorescence analysis of methanol-fixed HeLa, using GCLM antibody at 1:200 dilution.

Application

• Non-transfected (–) ａnd transfected (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, ａnd the membrane was blotted with GCLM antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Application

• Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, ａnd the membrane was blotted with GCLM antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Application

• Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, ａnd the membrane was blotted with GCLM antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control

HeLa , A431 , mouse liver , rat liver , rat kidney

Application Notes

*Optimal dilutions/concentrations should be determined by the researcher.:

Additional Information

Form

Liquid

Storage Instructions

Keep as concentrated solution. Aliquot ａnd store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Storage Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.